Vol. 8 (4) : July-August 2017 issue
Green Farming Vol. 8 (4) : 814-818 ; July-August, 2017
Assessment of chickpea (Cicer arietinum L.) cultivars using PCR based molecular markers
R.E. BHANDIGARE1, S.S. SHENDE2*, D.H. WAGHMARE3 and A.M. DESHMUKH4
Department of Plant Biotechnology, Vilasrao Deshmukh College of Agricultural Biotechnology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Latur - 413 512 (Maharashtra)
Designation : 1,4P.G. Student, 3Junior Research Fellow, 2Associate Professor & Head *(sarikasshende@gmail.com)
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-5687
Total Pages : 5
Received : 12 August 2016
Revised accepted : 16 July 2017
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Citation :
R.E. BHANDIGARE, S.S. SHENDE, D.H. WAGHMARE and A.M. DESHMUKH. 2017. Assessment of chickpea (Cicer arietinum L.) cultivars using PCR based molecular markers. Green Farming Vol. 8 (4) : 814-818 ; July-August, 2017
ABSTRACT
Genetic diversity analysis of chickpea germplasm can provide practical information for the selection of promising parental material and thus assist in designing the breeding programmes. In the present investigation, Random Amplified Polymorphic DNA (RAPD) marker and Inter Simple Sequence Repeats (ISSR) markers were used to study the genetic diversity of twenty chickpea genotypes. Amplification of genomic DNA of 20 genotypes, using 8 RAPD primers yielded 56 amplicons with 60.71% polymorphism. Total percent polymorphism by ISSR marker was 63.46%. Out of 20 genotypes, more diverse genotype shown by RAPD and ISSR marker is BGD-2048. Present investigation on genetic diversity analysis in chickpea germplasm showed that, RAPD and ISSR marker techniques are useful in accessing genetic diversity of chickpea germplasm.
Key words :
Chickpea germplasms, ISSR, Polymorphism, RAPD.
