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International Journal of Applied Agricultural & Horticultural Sciences
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DOI Prefix : 10.37322
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Vol. 7 (5) : September-October 2016 issue
Green Farming Vol. 7 (5) : 1073-1076 ; September-October, 2016
Assessment of morpho-molecular diversity in blackgram (Vigna mungo (L.) Hepper)
YASHODAa1*, T.H. GOWDAa2 and SHAILAJA HITTALMANIb3
aGenetics and Plant Breeding, College of Agriculture Sciences, Shimoga, University of Agricultural and Horticulture Sciences, Shimoga - 577 205 (Karnataka) bDeptt. of Genetics & Plant Breeding, College of Agriculture Sciences, UAS, Bangalore – 560 065 (Karnataka)
Designation :  
1Ph.D. Scholar *(yj31990@gmail.com), 2Director (Extention), 3Professor and Head
Subject : Crop Genetics and Plant Breeding
Paper No. : P-4437
Total Pages : 4
Received : 31 October 2015
Revised accepted : 04 August 2016
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Citation :

YASHODA, T.H. GOWDA and SHAILAJA HITTALMANI. 2016. Assessment of morpho-molecular diversity in blackgram (Vigna mungo (L.) Hepper). Green Farming Vol. 7 (5) : 1073-1076 ; September-October, 2016

ABSTRACT
An investigation was conducted at KVK, UAHS, Shimoga to analyze the morphological and molecular (SSR) diversity of 46 black gram genotypes for 12 morphological characters during kharif 2013. Forty six genotypes of black gram were subjected to genetic divergence by using D² statistic. The genotypes were grouped into three clusters by D² analysis. Cluster I consisted maximum genotypes (44) and cluster II and III consisted only 1 genotype. The inter-cluster distances were greater than intra-cluster distances, revealing that less amount of genetic diversity existed among the genotypes. 100-seed weight, days to maturity, number of seeds per pod were major traits causing genetic divergence among genotypes. Cluster III had highest mean value for most of the traits. The genotypes M-186 and M- 191 may serve as potential parents for hybridization programme in the improvement of yield. Molecular markers are powerful tools for detecting diversity in genotypes. In present investigation 46 black gram genotypes were assessed for genetic diversity by using 11SSR primers. Results are revealed as among 11 primers the maximum number of alleles were observed for all the marker Ccmt 1, Ccmt 35, Ccmt 2, Ccmt 42, Ccmt 44, Ccmt 49, Ccmt 10, Ccmt 57, Ccmt 36, Ccmt 52 (2) except for Ccmt 5 (1). Maximum gene diversity was observed for Ccmt 1 (0.4969) followed by Ccmt 2 (0.4764) and Ccmt 35 (0.3856). Highest polymorphic information content (PIC) value was observed for the primer Ccmt1 (0.37) followed by Ccmt 2 (0.36). Maximum allele frequency was noticed in primer Ccmt5 (1.00), Ccmt 49(0.91) followed by Ccmt 44 (0.84). The UPGMA based dendrogram was obtained from the data deduced from the DNA profiles of the samples analyzed. Jaccard's similarity coefficients which ranged from 22 to 100 per cent and at similarity co-efficient of 24 per cent, seven main groups were observed. Maximum number of genotypes were observed in cluster II (14) followed by cluster III (13).
Key words :
Blackgram, D² statistic, Jaccard's similarity coefficients, Morphological characters, SSR markers, Vigna mungo.