Vol. 8 (3) : May-June 2017 issue
Green Farming Vol. 8 (3) : 506-511 ; May-June, 2017
Developing improved versions of a popular rice variety (Improved White Ponni) through marker assisted backcross breeding
MUTHUKUMAR M.a1, SASIKALA R.b2, S. ROBINc3 and M. RAVEENDRANa4*
aDepartment of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, cDeptt. of Rice, Centre for Plant Breeding and Genetics, Tamil Nadu Agric.University, Coimbatore - 641 003 (T.N.)
bDryland Agricultural Research Station, Chettinad, Karaikudi, Dist. Sivaganga - 630 102 (Tamil Nadu)
1Ph.D. Scholar, 2Asstt. Professor, 3,4Professor *(firstname.lastname@example.org)
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-5730
Total Pages : 6
Received : 22 August 2016
Revised accepted : 08 March 2017
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MUTHUKUMAR M., SASIKALA R., S. ROBIN and M. RAVEENDRAN. 2017. Developing improved versions of a popular rice variety (Improved White Ponni) through marker assisted backcross breeding. Green Farming Vol. 8 (3) : 506-511 ; May-June, 2017
Drought is the prime devastating abiotic stress limiting rice production in lowland and upland ecosystems. Developing improved versions of ruling rice varieties with enhance drought tolerance trait is a promising approach for introgression of large effect QTLs from drought tolerant donors through molecular assisted backcross breeding (MABB). In the present study, a set of 3 backcross inbred lines (BILs) harboring 2 or 3 large effect QTLs controlling grain yield under drought stress (viz., qDTY 1.1, qDTY 2.1, qDTY 3.1) from Apo, an upland drought tolerant rice variety harboring these target QTLs were developed in the genetic background of Improved White Ponni (IWP), a drought susceptible, fine grain type, popularly cultivated variety in Tamil Nadu, India through marker assisted QTL pyramiding. Three superior lines of BC2F3 generation of IWP x Apo population (BILs) were selected by foreground selection using SSR markers linked with these 3 target QTLs and background selection using 73 genome wide polymorphic SSR markers. Recipient parent IWP genome recovery in the range of 82.57 – 87.05% was recorded in the BILs. These selected BILs were categorized into three classes based on the QTL combinations which comprises of i) BIL # 4-7-4-24 in Class A (qDTY 1.1 + qDTY 2.1 + qDTY 3.1), ii) BIL # 10-1-1-5 in Class B (qDTY 1.1 + qDTY 3.1) and iii) BIL # 10-1-1-10 in Class C (qDTY 1.1 + qDTY 2.1). All the 3 selected BILs were evaluated for their phenotypic performance under drought stress conditions in two summer seasons (Jan 2015 and Jan 2016); which revealed that all 3 BILs performed better over the recipient parent in terms of grain yield and also its secondary traits like days to flowering, spikelet fertility percentage and plant height. This study proves that enhanced drought tolerance can be achieved through marker assisted backcross breeding approach by pyramiding large effect qDTY regions from Apo.
Key words :
Drought tolerance, Improved White Ponni, Marker assisted backcross breeding, Near isogenic lines, Rice.