A field experiment was conducted during 2014-15 under LTFE at JNKVV, under soybean-wheat crop sequence with 8 treatments viz., 50%, 100%, 150% NPK, 100% NP, 100% N, 100% NPK+FYM, 100% NPK-S and Control, replicated with four times in randomized block design. The successive addition of S fertilizers increased the available S and S pools from its initial status and highest deposition (42.66 kg ha-1) in the treatment receiving 100% NPK+FYM followed by 150% NPK (39.30 kg ha-1) addition, while the lowest S status was recorded in control (13.95 kg ha-1) followed by where continuous use of N alone fertilizers and S free fertilizers. The result on relationship between pools of S and availability of S showed that highly significant further, S pools were significantly correlated with yield of soybean. The highest crop yield was recorded with balanced NPK applied with FYM (1300 kg ha-1). The finding of the experiment revealed that the abundance of various forms of S was in the following decreasing order: total S>organic S>heat soluble S>available S> water soluble S.