The present investigation was carried out in the experimental field of Division of Fruit Science with a view to study the various physical and biochemical changes that occur during cold storage conditions by preharvest application of various chemicals. Eight year old plants of peach cv. Shan-i-Punjab were sprayed with naphthalene acetic acid (10,20 and 30 ppm), gibberellic acid (10,20 and 30 ppm) and TIBA (5,10 and 15 ppm). Fruits from treated plants were picked at physiological mature stage, then the fruits were immediately carried to the laboratory for sorting and packaging in CFB boxes and then stored in cold store (0 to 3.3°C temperature and RH 85-90%). The fruits were analyzed at different storage intervals i.e. 0,5,10, 15,20 and 25 days after storage. Maximum yield 81.2 kg/tree, minimum physiological loss in weight 0.03% were recorded in fruits treated with TIBA 10 ppm. Maximum total soluble solids 12.55%, total sugars 7.83% were recorded in fruits treated with gibberellic acid 30 ppm. Firmness of fruits 4.16% decreased with the advancements of storage periods.