Fruits of five different mulberry varities viz., Enshutakasuke, Rokokayasu, Koksu-21, Botatul and Brentul were analyzed for proximate composition during refrigerated storage conditions. Ripe fruits were collected from Mulberry Germplasm Bank of Temperate Sericulture Research Institute, SKUAST-K, Mirgund, J&K. During refrigerated storage of 30 days various parameters viz., Physiological loss of weight in fruits, acidity, total soluble solids, total sugar, reducing sugars, proteins, vitamin C, total phenolics as anthocyanins were studied at the intervals of 5 days. The results showed that under refrigerated storage of 30 days, there was gradual increase in physiological loss of weight from 2.22 to 21.73 per cent while as titrable acidity decreases from an initial value of 0.31 to 0.04 per cent. Total soluble solids, total sugars and reducing sugars increased from 22.27 to 26.58 per cent, 20.26 to 21.89 per cent and 16.69 to 18.20 per cent respectively. Vitamin C, total phenols as anthocyanins and protein content decreased from 22.49 to 14.11mg/100g, 129.48 to 121.83mg/100g and 0.72 to 0.47 per cent respectively.