Soil health or soil fertility depends on physical, chemical and biological characteristics as these characteristics improve water and nutrient extraction and utilization potential of the plants. Organic farming is the most common practise in tackling farming challenges and issues and is also considered as the sustainable way of practicing agriculture. In order to establish this, spinach (Spinacea oleracea) was grown using three different soils namely Amrutmitti, IIT nursery soil and Chemi-Agri soil (taken from railway farms) under open-field conditions for 40 day cycle. To conduct this study, parameters used for assessing plant performance are; yield of spinach, physical properties such as; plant height, plant weight, stem diameter, petiole and leaf characteristics and finally nutritional quality such as; vitamin A, vitamin C and phenolic content. Soil and field experiment revealed that organic soils prepared in IIT nursery soil gave the highest spinach yields with 62%. Further nutrition analysis showed that spinach grown in organic soils (IIT nursery soil) was higher in phenolic content and vitamin content. However, spinach grown on Amrutmitti had 56% higher Vitamin C content as compared to USDA standards and highest among crops from other soils. Thus, it was recommended that using organic soil with well-maintained conditions gives higher yield of leafy vegetables in an economic and sustainable way.