Genetic divergence analysis, using Mahalanobis D2 statistics, was carried out in thirty indigenous cucumber genotypes for twenty four characters. All the character under study showed considerable divergence and the genotypes were grouped into five clusters. The clustering pattern had no parallelism between genetic diversity and geographical distribution, suggesting that the selection of parental genotypes for hybridization will be more appropriate based on genetic diversity. Cluster III contained the maximum (15) number of genotypes, whereas cluster I contained minimum (2) genotypes. The genetic stocks within a cluster had smaller D2 values among themselves than those belonging to different clusters. Intra-cluster distance was maximum (3.123) in cluster II whereas, it was minimum (1.475) in cluster I. Maximum average inter-cluster distance (10.755) was recorded between cluster II and cluster V, suggesting the greater chances of getting superior hybrids in F1 or transgressive segregants in subsequent generations. Genotypes in cluster I was superior in seed germination and had minimum seed cavity length and breadth and PDI. Cluster IV had superiority in terms of average fruit weight, fruit length and seed cavity length. Cluster V recorded highest mean value for most of the characters. Considering the diversity pattern and mean performances, the genotypes LC-20 and LC-21 form cluster I, LC-7 from cluster III, LC-28 from cluster IV and LC-2, LC-9, LC-10 and LC-16 from cluster V would be best choice as parents for future hybridization programs.