Genetic divergence or the presence of substantial genetic variation in the breeding population is one of the prerequisite for genetic gain in any crop improvement program. Therefore, the present study was attempted to assess the genetic variation present in advance soybean genotypes for different agronomic traits which can be used to decide a sound breeding program for genetic improvement in this crop. A set of fifty advance genotypes of soybean were evaluated in completely Randomized block design with three replications at College of Agriculture, Jabalpur, India during rainy 2014-15. The results revealed significant genotypic variation for all the traits under study except number of primary branches per plant, pod length and seeds per pod. Diversity study among the genotypes revealed that 50 genotypes were grouped into 10 clusters. Cluster I having highest number of genotypes (14) followed by cluster II (12) and III (10). Maximum contribution towards divergence was reported for growing degree days followed by seed yield per plant and days to maturity. High heritability coupled with high genetic advance as per cent of mean was observed for number of pods per plants, hundred seed weight, seed yield per plant and harvest index indicates that improvement in these traits can be done by selecting superior plants. The diversity in early flowering, maturity and yield can be exploited for breeding early maturing varieties with higher yield.