Vol. 8 (4) : July-August 2017 issue
Green Farming Vol. 8 (4) : 802-807 ; July-August, 2017
Genotypic and phenotypic evaluation of parents for phosphorus starvation tolerant gene PSTOL1 in rice for marker assisted selection
K. CHITHRAMEENAL1*, M. RAVEENDRAN2 and J. RAMALINGAM3
Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore - 641 003 (T.N.)
1Ph.D. Scholar *(email@example.com), 2,3Professor
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-6460
Total Pages : 6
Received : 11 January 2017
Revised accepted : 22 July 2017
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K. CHITHRAMEENAL, M. RAVEENDRAN and J. RAMALINGAM. 2017. Genotypic and phenotypic evaluation of parents for phosphorus starvation tolerant gene PSTOL1 in rice for marker assisted selection. Green Farming Vol. 8 (4) : 802-807 ; July-August, 2017
Phosphorus which is a key element for plant growth is found to be very limited in soil and also most of the commercial modern cultivars have low phosphorus use efficiency. So phosphorus use efficient crops have to be developed. This can be better accomplished by the utilization of molecular tools in addition to field breeding programmes. A quantitative trait locus Pup1 (phosphorus uptake 1) for phosphorus deficiency tolerance was identified in an aus-type traditional variety Kasalath about a decade ago. By subsequent sequencing of the region, a protein kinase gene PSTOL1 (Phosphorus starvation tolerant 1) responsible for the trait P use efficiency was identified. This gene is absent in rice reference genome and phosphorus use inefficient modern cultivars. We planned to introgress PSTOL1 gene in commercial varieties of South India through backcross breeding. For that we evaluated both the donors and the recipients for the presence of PSTOL1 gene. Kasalath was used as positive control. When genotyping was done with gene specific molecular marker, the NILs introgressed with Kasalath alleles which we planned to use as donors, were clearly differentiated from the modern varieties for P use efficiency due to presence of the PSTOL1 gene. Also the modern varieties which were improved for blast and bacterial blight diseases resistance were checked for the presence of the resistance genes through the use of concerned gene specific markers. By combining the P use efficiency of the NILs with the high yield efficiency and multiple disease resistance of the modern varieties, it is possible to increase the grain yield of modern commercial varieties even under P deficient conditions.
Key words :
Genotypic difference, Molecular marker, Phosphorus use efficiency, PSTOL1, Rice.