Vol. 8 (3) : May-June 2017 issue
Green Farming Vol. 8 (3) : 535-540 ; May-June, 2017
Identification and validation of SSR markers after phenotypic characterization in M2 mutant population of tomato
T. HARITHA1*, V. SATYANARAYANA RAO2, LAL AHAMED M.3 and Y. ASHOKA RANI4
Department of Genetics and Plant Breeding, Agricultural College, Bapatla - 522 101, Dist. Guntur (Andhra Pradesh)
1Ph.D. Scholar *(firstname.lastname@example.org), 2,4Professor, 3Asstt. Professor
Subject : Crop genetics & Plant breeding
Paper No. : P-6034
Total Pages : 6
Received : 01 September 2016
Revised accepted : 27 February 2017
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T. HARITHA, V. SATYANARAYANA RAO, LAL AHAMED M. and Y. ASHOKA RANI. 2017. Identification and validation of SSR markers after phenotypic characterization in M2 mutant population of tomato. Green Farming Vol. 8 (3) : 535-540 ; May-June, 2017
Genetic variation for phenotypic characters occurs both within cultivated tomato and wild species of tomato. The study of such agro-morphological variability is the traditional method of assessing genetic diversity. Such characterization, however, is often hindered by the limited number of polymorphic features and the need for multiple test environments. Considering the wide range of implications of molecular markers the present work was undertaken to characterize 1% EMS mutagen treated 109 M2 lines (along with control) for which phenotypic variations were observed using reported simple sequence repeat markers. A total of 26 SSR markers randomly selected from the Solanaceae Genome Network and Tomato Mapping Resource Database were selected for validation of earlier reported traits in tomato in the present 1% EMS treated mutant population. Of the 26 primers screened, only two SSRs namely SSR 103 and SSR 383 are polymorphic with PIC values each of 0.51 making them informative. Similarity coefficient based on DNA amplification by SSRs varied from 0.48-1.00. Cluster analysis grouped 109 M2 mutant lines along with control into two major clusters at 92 % similarity level. Based on the similarity coefficient two mutant lines viz., e13-4 (from cluster I) which showed elongated fruits and e213-8 (from cluster IIB) which showed dark green colour of immature fruit are found to be diverse. So, these two mutant lines may serve as valuable genetic stocks in future and hybridization between these distantly related mutant lines may realize mutant lines with variation in fruit colour or shape. On, further studies this may help to map QTLs responsible for this phenotypic variation.
Key words :
Cluster analysis, Ethyl methane sulfonate, Simple sequence repeats, Tomato, Validation.