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International Journal of Applied Agricultural & Horticultural Sciences
  • 28 March, 2024
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Frequency : Bimonthly
Language : English
DOI Prefix : 10.37322
P-ISSN : 0974-0775
E-ISSN : 2582-4198
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Vol. 7 (2) : March-April 2016 issue
Green Farming Vol. 7 (2) : 300-304 ; March-April, 2016
Molecular characterization of nitrogen fixing Ensifer species from Vigna trilobata growing in alkaline soil of Thar Desert
ALKESH TAKa1, NISHA TAKa2, INDU SINGH SANKHLAb3, RAJU RAM MEGHWALa4 and HUKAM S. GEHLOTa5*
aBNF and Stress Biology Lab, Department of Botany, Jai Narain Vyas University, Jodhpur - 342 033 (Rajasthan)
bDepartment of Botany, University of Rajasthan, Jaipur - 302 004 (Rajasthan)
Designation :  
1,2,3Asstt. Prof., 4Post-Doc. Fellow, 5Professor *(hsgehlot@gmail.com)
Subject : Agriculture Botany
Paper No. : P-4603
Total Pages : 5
Received : 25 December 2015
Revised accepted : 03 March 2016
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Citation :

ALKESH TAK, NISHA TAK, INDU SINGH SANKHLA, RAJU RAM MEGHWAL and HUKAM S. GEHLOT. 2016. Molecular characterization of nitrogen fixing Ensifer species from Vigna trilobata growing in alkaline soil of Thar Desert. Green Farming Vol. 7 (2) : 300-304 ; March-April, 2016

ABSTRACT
Thirty four bacterial strains were isolated from root nodules of native legume Vigna trilobata growing in the alkaline soil of Thar Desert. Phenotypically and genetically diverse bacterial strains were grouped on the basis of ARDRA and RAPD patterns. A representative strain from each RAPD group was chosen for further molecular characterization. 16S rRNA gene of three bacterial strains (VT3, VT9 and VT29) were sequenced and identified as Ensifer sp. which were 100% similar to each other and formed a separate clade from type strain Ensifer saheli LMG 7837T with maximum sequence similarity of 99.5%. The symbiotic genes nodA and nifH of Ensifer strain VT29 was sequenced which showed incongruence with 16S rRNA gene phylogeny. The V. trilobata plants inoculated with isolated strains of Ensifer showed higher chlorophyll contents and biomass in glass house experiment. The present study suggested that V. trilobata is nodulated by fast growing species of Ensifer that are effective in increasing growth of host plants and are well adapted to alkaline soil of arid and semi-arid regions of Indian Thar Desert.
Key words :
Ensifer, Phylogeny, Symbiotic gene, Thar Desert, Vigna trilobata.