Vol. 8 (5) : September-October 2017 issue
Green Farming Vol. 8 (5) : 1056-1061 ; September-October, 2017
Molecular detection of rust resistant gene Lr34/Yr18 and quality genes in Indian wheat varieties
ANJALI RAIab1*, DEEPAK GANJEWALAa2, SUMIT K SINGHb3, TEJ PRATAP JITENDRA KUMARb4, POORNIMA SHARMAb5, NEELU JAINb6 and ANJU M. SINGHb7**
aDepartment of Biotechnology, Amity Institute of Biotechnology, Amity University, Noida - 201 313 (Uttar Pradesh)
bDepartment of Genetics, Indian Agricultural Research Institute, Pusa - 110 012 (New Delhi)
1,3,4SRF *(email@example.com), 2Asstt. Professor, 5RA, 6Sr. Scientist,
7Principal Scientist **(firstname.lastname@example.org)
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-6924
Total Pages : 6
Received : 19 July 2017
Revised accepted : 30 August 2017
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ANJALI RAI, DEEPAK GANJEWALA, SUMIT K SINGH, TEJ PRATAP JITENDRA KUMAR, POORNIMA SHARMA, NEELU JAIN and ANJU M. SINGH. 2017. Molecular detection of rust resistant gene Lr34/Yr18 and quality genes in Indian wheat varieties. Green Farming Vol. 8 (5) : 1056-1061 ; September-October, 2017
Rusts are among the most important fungal diseases of wheat worldwide and cause between 20-40% of yield losses annually. Resistance breeding to overcome the threat posed by the rust pathogens has been considered as the most practical and durable approach in wheat. One such gene for leaf rust resistance is Lr34 closely linked with stripe rust resistance gene Yr18 and a stem rust resistance gene Sr57. Lr34, known as adult plant resistance genehas been introduced in Indian wheat germplasm from several sources especially the CIMMYT derived germplasm. A molecular marker csLv34 was used to screen sixty seven wheat varieties released in the last two decades to ascertain the presence of Lr34 gene. 24 wheat varieties showed the amplification of the marker. The results were also corroborated with Average Coefficient of Infection data of these varieties. This information will be useful to the breeders to assess the presence of the genes Lr34/Yr18/Sr57 during the development of new germplasm. Molecular typing for the quality genes- PinA, HMW-GS was done in Indian genotypes. Therefore, screening the combination of HMW-GS and PINA gene in Indian genotypes will generally benefit breeding efforts for quality improvement.
Key words :
Adult plant resistance gene, Average Coefficient of Infection (ACI), csLV34, HMW-GS, Leaf rust resistance Lr34, PINA, Wheat varieties.