The present investigations were conducted to evaluate the effect of storage condition on the pollen viability and germination in Rangpur lime (Citrus limonia Lush.), Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf], Trifoliate orange [Poncirus trifoliata (L.) Raf] and Swingle citrumelo [Citrus paradisi Macf. x Poncirus trifoliata (L.) Raf.]. The pollen were stored at three temperatures viz., -80°, -20° C and room temperature. Highest mean pollen viability (36.06 %) and germination (27.02 %) was observed in trifoliate orange which was significantly higher from all other species irrespective of storage period time. The pollen viability and germination decreased significantly with the increase in period of storage at room temperature in all the rootstock genotypes which was recorded maximum in first 10 days of storage. In all the rootstock genotypes the highest mean pollen viability and germination were recorded at -80°C followed by-20°C and minimum at room temperature, irrespective of storage interval. The maximum pollen viability of 66.46 per cent (at-20°C ) and 72.26 per cent (at -80°C) was recorded in trifoliate orange which was at par with pollen viability of Carrizo citrange and differed significantly from all other rootstock genotypes, whereas the minimum pollen viability (57.05 %) was recorded in Rangpur lime. Similarly, maximum pollen germination 46.57 per cent (at -20°C) temperature and 72.26 per cent (at -80°C) was recorded.