Lack of seed testing protocols causes hindrance in tests for germination and vigour parameters of Panicum miliaceum seeds and ultimately results in adverse effect of marketing To determine standard germination of a seed lot germination test was carried out under a given set of conditions, namely suitable substratum, optimum temperature and sufficient quantity of water to germinating seeds Seed germination of proso millet was significantly affected by substratum. The interaction effect of substratum and temperature were significant for speed of germination, standard germination root and shoot length along with seed vigour. The highest speed of germination (86.00%) was recorded in top of the paper. This method was significantly superior between the paper method. Higher germination was recorded at 25?C followed by 20?C which was statistically at par with each other but significantly higher than that at 30?C and 35?C. Besides speed of germination, treatments comprising 25±01C temperature with between paper method recorded maximum root growth, seedling fresh weight and seed vigour (6.55cm, 0.078mg and 1365.65 respectively) than the remaining treatments. Thus, it could be concluded that a top and between method with 25o temperature is recommended for seed germination and seed vigour index.