Vol. 7 (5) : September-October 2016 issue
Green Farming Vol. 7 (5) : 1034-1039 ; September-October, 2016
Whole plant regeneration through direct organogenesis in sesame (Sesamum indicum L.)
SHILPA V. MALAGHAN1*, R. LOKESHA2 and SAVITHA REVADI3
Plant Tissue Culture and Molecular Laboratory, Department of Genetics & Plant Breeding, College of Agriculture, PB No. 329, University of Agricultural Sciences, Raichur – 584 102 (Karnataka)
Designation : 1Ph.D. Scholar *(malaghan4471@gmail.com), 2Professor & Head, 3Technical Asstt.
Subject : Crop Genetics and Plant Breeding
Paper No. : P-4612
Total Pages : 6
Received : 29 December 2015
Revised accepted : 17 August 2016
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Citation :
SHILPA V. MALAGHAN, R. LOKESHA and SAVITHA REVADI. 2016. Whole plant regeneration through direct organogenesis in sesame (Sesamum indicum L.). Green Farming Vol. 7 (5) : 1034-1039 ; September-October, 2016
ABSTRACT
Whole plant regeneration, hardening and field transfer of sesame (Sesamum indicum L.), through de-embryonated cotyledons exercised from seeds of six genotypes were studied. Explants were inoculated on to ½ MS media supplemented with 20 µM TDZ+2.5 µM IAA along with (25µM BAP) and without BAP. In addition MS with 25µM BAP alone was also tried. Regeneration ability of genotypes was promising on ½ MS media with TDZ+IAA and with (81.44%) or without BAP (80.36%). No regenerability (<5%) was observed in MS media with 25µM BAP alone. The genotypes used in study showed significant difference for shoot regeneration ability, shoot number per ex-plant and shoot length. Well developed in vitro shoots were transferred to MS+3%Sucrose+2.7?M of ?-naphthalene acetic acid (NAA) for root induction. The rooted plantlets were transplanted to soil after hardening. Only four plants, one of DS-1 and three of W-II (8.25% of total plants) survived, flowered and produce seeds.
Key words :
Growth regulators, Regeneration, Rooting and hardening, Sesamum indicum.
