DNA polymorphism in sugarcane woolly aphid, Ceratovocuna lanigera Zehnter (Aphididae : Homoptera) through RAPD markers

Green Farming Vol. 1 (1) : 74-77 (Jan.-Feb., 2010) (Renewed)

P.V. MATTI,   C.P. MALLAPUR,  R.J. RABINDRA  and  D.P. BIRADAR

Department of Agric. Entomology, University of Agricultural Sciences, Dharwad - 580 005 (Karnataka)

Project Directorate of Biological Control (ICAR), Hebbal, Bangalore - 560 024 (Karnataka)

ABSTRACT .

RAPD technique helps to identify discreet genetic markers in Aphididae, a family in which biochemical and morphological genetic polymorphisms are rare. Investigations were carried out to study the genetic diversity through, molecular characterization of sugarcane woolly aphid (SWA) under laboratory conditions. RAPD revealed large amount of genetic variations among individuals. There exist variations in the DNA profiling among all samples. The dendrogram constructed using symmetric matrix of different samples resulted into two major clusters. The similarity matrix pertaining to different isolates of SWA revealed that the similarity coefficient ranged from 0.22-0.60. The highest genetic similarity index of 0.60 was seen between the isolates from Bangalore and Poona followed by 0.40 between the isolates from Assam, Sameerwadi (Bagalkot).