Vol. 11 (4 & 5) : July-October 2020 Issue
Green Farming Vol. 11 (4 & 5) : 408-410 ; July-October 2020
Influence of microspore developmental stages on callus induction in Indica rice (Oryza sativa L.) varieties IR 28 & IR 66
R.U. SOLANKIa1* and K.B. KATHIRIAb2
a.Medicinal & Aromatic Plants Research Station, b.College of Food Processing Techn. & Bio Energy, Anand Agricultural University, Anand - 388 110 (Gujarat)
Designation : 1Research Associate *(rajpalsinhsolanki9@gmail.com), 2Principal & Dean
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. :
Total Pages : 3
Received : 13 July 2020
Revised accepted : 13 August 2020
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Citation :
Solanki, R.U. and Kathiria, K.B. 2020. Influence of microspore developmental stages on callus induction in Indica rice (Oryza sativa L.) varieties IR 28 & IR 66. Green Farming Vol. 11 (4 & 5) : 408-410 ; July-October, 2020.
ABSTRACT
Success of rice anther culture experiment greatly depends on the stage of microspore development at the time of inoculation. Present study was aimed at deciding the appropriate stage of anther development which can record higher callus induction percentage in both the indica rice varieties IR 28 and IR 66. Inoculation of rice anthers at uninucleate stage of microspore development had irrefutable influence on the per cent callus induction in both the varieties IR 28 and IR 66 providing superior callusing (6.50 and 5.00 per cent, respectively) and moderate size of the callus (2.09 and 1.88 mm3, respectively) after 12 weeks of inoculation. Size of the callus on the other hand, was found better with the advanced (binucleate) stage of anther development.
Key words :
Anther development, Callus induction, Indica rice, Microspore development.
