Vol. 8 (3) : May-June 2017 issue
Green Farming Vol. 8 (3) : 518-522 ; May-June, 2017
Assessment of genetic diversity and genetic purity in the elite rice variety, Samba Mahsuri using SSS markers
P. SRAVANTHI1*, CH. V. DURGA RANI2, R.M. SUNDARAM3, S. NAGALAKSHMI4, S. SIVARAMAKRISHNAN5 and S. VANISREE6
Department of Agricultural Biotechnology, College of Agriculture, Acharya N.G. Ranga Agricultural University, Rajendranagar, Hyderabad - 500 030 (Andhra Pradesh)
1Research Scholar *(firstname.lastname@example.org), 2Professor, 3Sr. Scientist, 4Research Fellow, 5Professor & Head, 6Professor
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-6101
Total Pages : 5
Received : 02 November 2016
Revised accepted : 06 May 2017
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P. SRAVANTHI, CH. V. DURGA RANI, R.M. SUNDARAM, S. NAGALAKSHMI, S. SIVARAMAKRISHNAN and S. VANISREE. 2017. Assessment of genetic diversity and genetic purity in the elite rice variety, Samba Mahsuri using SSS markers. Green Farming Vol. 8 (3) : 518-522 ; May-June, 2017
Forty two fine grain rice varieties released from ANGRAU were studied in order to determine the molecular identity of Samba Mahsuri by using Genomic and EST SSR markers. Forty SSR primers amplified a total of 96 alleles with an average of 2.4 alleles per marker, RM 11278 was able to distinguish BPT 5204 from the remaining popular varieties by amplifying an allele of 270 bp. This primer was considered as a molecular ID for BPT 5204. Cluster analysis based on Jaccard's similarity coefficient using UPGMA grouped the 42 rice varieties into seven clusters. The clustering pattern was observed to be mainly based on the grain type and genetic background of the varieties. To utilize these SSR markers specific for Samba Mahsuri effectively for detection of impurities in Samba Mahsuri, a rapid and cost effective two-dimensional bulked DNA sampling strategy was designed and validated. The SSR marker RM 11278 was found to be an efficient tool in the process of quality control of BPT 5204.
Key words :
Dendrogram, EST-SSRs, Genetic purity, Jaccord similarity coefficient, Samba Mahsuri.