Vol. 6 (2) : March-April 2015 issue
Green Farming Vol. 6 (2) : 259-262 ; March-April, 2015
Assessment of molecular diversity analysis in muskmelon (Cucumis melo L.) genotypes in relation to drought tolerance
S. MISHRAa1*, A.K. SHARMAa2, D. SHARMAb3, R.S. SHARMAb4 and R.P. MEENAc5
aDept. of Plant Breeding & Genetics, College of Agric., S.K. Rajasthan Agricultural University, Bikaner - 334 006
bDept. of MBBT, Rajasthan College of Agric., M.P. University of Agricultural & Techn. , Udaipur - 313 001 (Rajasthan),
cNational Research Centre on Plant Biotechnology, Indian Agric. Res. Institute, Pusa, New Delhi - 110 012
Designation : 1,3,4,5Ph.D. Scholar *(radhebiotech88@gmail.com), 2Professor
Subject : Crop Genetics and Plant Breeding
Paper No. : P-2053
Total Pages : 4
Received : 23 May 2014
Revised accepted : 03 January 2015
First Page
Citation :
S. MISHRA, A.K. SHARMA, D. SHARMA, R.S. SHARMA and R.P. MEENA. 2015. Assessment of molecular diversity analysis in muskmelon (Cucumis melo L.) genotypes in relation to drought tolerance. Green Farming Vol. 6 (2) : 259-262 ; March-April, 2015
ABSTRACT
Molecular markers are widely used tools in genotypic diversity analysis and species identification. Molecular diversity was performed with 15 RAPD primers among 18 diverse accessions (9 drought tolerant and 9 susceptible) of muskmelon. Out of fifteen ten RAPD primers produced 45 amplified DNA fragments ranging in size from 200–2000 base pairs, from which 38 fragments were polymorphic (83.33%) with an average of 4.5 fragments per primer. Six primers were found to be highly informative as they gave unique bands to differentiate between drought susceptible and drought tolerant genotypes. From RAPD profiles similarity matrix was obtained and Jaccard's Similarity Coefficient was observed in between 0.45 to 0.86 and on this basis a dendogram was constructed with UPGMA method. This study demonstrates that RAPD are highly useful for finding markers associated with major and minor genes controlling agronomically important traits in muskmelon.
Key words :
Drought tolerance, Muskmelon genotypes, Molecular diversity, Polymorphism, RAPD.