Escalating fuel prices with enhanced level of green house gases and increased demand for fuel have challenged many researchers to ponder over environment friendly alternative fuel by using different feedstocks and waste water resources as a growing medium. The objective of the present study was to assess the efficiency of selected microalgal genera to remove nitrogen and phosphorus from secondary treated sewage waste waters. The idea was also to ascertain whether such waters can be used for the growth of these with subsequent utilization of the biomass in the area of biofuel or value addition. The three microalgal genera namely Chlamydomonas sp., Chlorococcum sp. and Chlorella sp. were able to grow in waste waters collected from two sites namely Nirankari Coronation Pillar Plant and Okhla Sewage Treatment Plant, Delhi during rainy, summer and winter seasons of 2013-2014. These microalgal genera exhibited their suitability to remove nutrients from such waters showing the potential in the area of bioremediation as well.