Vol. 4 (4) : July-August 2013 issue
Green Farming Vol. 4 (4) : 489-492 (July-August, 2013)
Biopriming of cucumber seeds with Trichoderma harzianum and Pseudomonas fluorescens for enhanced plant defense against Pythium aphanidermatum
T. SIVASANKARI DEVIa1* and M.K. SHIVAPRAKASHb2
aDepartment of Agricultural Microbiology, TNAU, Coimbatore – 641 003 (Tamil Nadu),
bDeptt .of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bangalore – 560 065 (Karnataka)
Designation : 1Research Fellow *(shank_pts@yahoo.co.in), 2Professor and Head
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-0681
Total Pages : 4
Received : 02 December 2012
Revised accepted : 28 May 2013
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Citation :
T. SIVASANKARI DEVI and M.K. SHIVAPRAKASH. 2013. Biopriming of cucumber seeds with Trichoderma harzianum and Pseudomonas fluorescens for enhanced plant defense against Pythium aphanidermatum. Green Farming Vol. 4 (4) : 489-492 ; July-August, 2013
ABSTRACT
The study was conducted to evaluate the efficacy of seed biopriming with Trichoderma harzianum (NBAII-TH10) and Pseudomonas fluorescens (NBAII-Pf1) in enhacing the production of defense enzymes PAL, PO, PPO for controlling damping-off of cucumber (Cucumis sativus L.) caused by Pythium aphanidermatum. Seed biopriming enhanced the efficiency of biocontrol agents, which increased the production of enzymes to protect the plant against Pythium. The level of enzymes was estimated at 3, 5 and 7 days after inoculation of pathogen. PAL, PO and POP levels was increased at 7 DAI in treatments were both Trichoderma harzianum (NBAII -TH10) and Pseudomonas fluorescens (NBAII -Pf1) were used for bio-priming along with PGPRs.
Key words :
Biopriming, Cucumber, Plant defense enzymes, Pseudomonas, Pythium, Trichoderma.
