Vol. 1 (6) : November-December 2010 issue
Green Farming Vol. 1 (6) : 645-648 (November-December, 2010) (New Series)
Characterization of milk clotting cysteine protease of Euphorbia nivulia Buch.-Ham. latex
SHAMKANT B. BADGUJARa1* and RAGHUNATH T. MAHAJANb2
aDepartment of Biotechnology, SSBT's, College of Engineering and Technology, Bambhori, Jalgaon - 425 001,
bDepartment of Biotechnology, Moolji Jaitha College, Jalgaon - 425 002 (Maharashtra)
Designation : 1Lecturer (Biotechnology), 2Reader (Zoology) & Head (Biotechnology)
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. :
Total Pages : 4
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Revised accepted :
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Citation :
SHAMKANT B. BADGUJAR and RAGHUNATH T. MAHAJAN. 2010. Characterization of milk clotting cysteine protease of Euphorbia nivulia Buch.-Ham. latex. Green Farming Vol. 1 (6) : 645-648 ; November-December, 2010 (New Series)
ABSTRACT
Here we report, characterization of partially purified milk clotting cysteine protease enzyme (MCE) obtained from the latex of Euphorbia nivulia Buch.-Ham. MCE was partially purified by centrifugation and fractional precipitation with acetone. The optimum pH range for milk clotting activity was 6.0-6.5 and it exhibited optimal activity at 500C. The isoelectric point of MCE is 3.4. The clotting activity of the MCE was immensely enhanced by CaCl2. The MCE was activated by thiol compounds and inhibited by common thiol-blocking reagents, particularly mercuric chloride and iodoacetic acid. This indicates that the enzyme belongs to cysteine protease family.
Key words :
Cysteine protease enzyme, Euphorbia nivulia, Laticiferous plant, Milk coagulation.
