Vol. 3 (3) : May-June 2012 issue
Green Farming Vol. 3 (3) : 253-259 (May-June, 2012)
Chimeric Cry1AaIa5Ia5 gene confers protection against Helicoverpa armigera in Nicotiana tabacum
K. SUSHMITAa1* and B. RAMESHb2
aNational Research Centre on Plant Biotechnology, LBS Building, IARI, Pusa Campus, New Delhi - 110 012
bDepartment of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut - 250 004 (U.P.)
Designation : 1Senior Research Fellow *(sush_sush20@yahoo.com), 2Professor, H.O.D and Dean Faculty of Agriculture
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-0286
Total Pages : 7
Received : 04 May 2012
Revised accepted : 29 May 2012
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Citation :
K. SUSHMITA and B. RAMESH. 2012. Chimeric Cry1AaIa5Ia5 gene confers protection against Helicoverpa armigera in Nicotiana tabacum. Green Farming Vol. 3 (3) : 253-259 ; May-June, 2012
ABSTRACT
Cry1 delta-endotoxins of Bacillus thuringiensis are generally active against lepidopteran insects, but Cry1Aa have low levels of activity against Helicoverpa armigera. Here we report the transformation of tobacco with gene encoding Cry1AaIa5Ia5 hybrid toxin that has increased activity against this insect species. The in-silico analysis of Cry1Aa and Cry1AaIa5Ia5 genes was done to find the structural similarities and dissimilarities. Diversity was seen in length of loops and composition of domain II and domain III of Cry1Aa and Cry1AaIa5Ia5 toxins after multiple sequence alignment. Superposition of 3-D structure confirmed it further.
Key words :
Bacillus thuringiensis, Cry gene, domain, 3-D structure, Helicoverpa armigera, in-silico analysis.
