Epicuticular waxes (EW) on leaf surface reduce leaf temperature and transpiration and form a protective barrier between plant and their environment. There are many genes which are associated with EW biosynthesis and examining the relevance of these genes is essential for crop improvement towards drought resistance. Post-transcriptional gene silencing (PTGS) using Virus-Induced Gene Silencing (VIGS) vectors are found to be useful in understanding the functional relevance of epicuticular wax gene, cer1. To standardize the conditions for PTGS, VIGS vector with Phytoene desaturase gene was infiltrated into model plants and maintained at specific conditions. Further, Nicotiana benthamiana plants infiltrated with Agrobacterium harboring cer1 gene cloned both from Nicotiana benthamiana and tomato showed the wilting symptoms, which was further quantified by molecular and physiological assays. The results implicate that genes cloned either from heterogenous or endogenous plants would give similar results which indicate the relevance of specific EW genes by PTGS.