Vol. 6 (1) : January-February 2015 issue
Green Farming Vol. 6 (1) : 5-8 ; January-February, 2015
Combined and comparative analysis of RAPD, ISSR and microsatellite markers for phylogenetic studies in tobacco
DHARMENDRA PATIDARa1*, R.S. FOUGATb2, A.D. PATELa3 and VISHAL R. PATILb4
aBidi Tobacco Res. Station, bDept. of Agril. Biotechnology, Anand Agricultural Univ., Anand - 388 110 (Gujarat)
Designation : 1,4 Ph.D. Scholar *(dharmendrapbg@gmail.com), 2Professor & Head, 3Res. Scientist
Subject : Biotechnology, Molecular biology, Agriculture Microbiology and Cancer Res.
Paper No. : P-1800
Total Pages : 4
Received : 09 April 2014
Revised accepted : 20 December 2014
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Citation :
DHARMENDRA PATIDAR, R.S. FOUGAT, A.D. PATEL and VISHAL R. PATIL. 2015. Combined and comparative analysis of RAPD, ISSR and microsatellite markers for phylogenetic studies in tobacco. Green Farming Vol. 6 (1) : 5-8 ; January-February, 2015.
ABSTRACT
Comparative study of RAPD, ISSR and SSR markers analysis were used to establish genetic identities and evaluate genetic diversity among 17 tobacco cultivars. Among these markers, RAPDs generated 205 amplification products, out of which 146 were polymorphic, ISSRs produced 73 amplification products, out of which 65 were polymorphic and SSR produced 54 amplification products, out of which 51 were polymorphic. SSR fingerprinting detected more polymorphic loci (94.4 %) than the ISSR (89.00 %) and RAPD (89.53 %). Polymorphism information content (PIC) for each of the marker systems (0.71 for RAPD, 0.89 for ISSR and 0.37 for SSR) suggested that all the marker systems were diversely effective in determining polymorphisms. The dendrograms constructed using RAPD, ISSR and SSR marker systems were highly correlated with each other as revealed by high Mantel correlation ranged (r= 0.887 to 0.986). Present study suggests these three marker systems were efficient for genetic diversity studies in tobacco.
Key words :
Comparative analysis, Genetic parameters, ISSR, PIC, RAPD, SSR, Tobacco.
