Pleurotus mushroom is a heterothallic fungus showing tertapolar incompatibility and multiple allelium. In the present study with this knowledge of life cycle of Pleurotus species, the compatibility test were carried out in the laboratory by using 14 different edible and wild Pleurotus species viz., P1, P2, P3, P5, P6, P7, P8, P10, P11, P12, P14, P16, P20 and P21 by dual culture technique. The response of individual mycelial growth of different Pleurotus species was very slow and thin. Whereas, after mating of both the species in the mating zone the mycelial growth was thick, become fast and absolute white. The strain P1 and P20 were compatible with all strains tested. Further, strain P1 and P20 were found compatible with each other also. It was also found that out of 91 compatibility test/dual cultures, 78 had shown the compatibility whereas 13 were found incompatible. Mycelial growth and fruit body production of hybrid strains of P1 and P20 also recorded. In general it was observed that new hybrid cultures had given higher mycelial growth as compared to their parent cultures. Further, it was also obseved that most of new hybrid strains produced fruit body in culture media.