Pseudomonas fluorescens is effective bacterial antagonists for the management of soil borne fungal pathogens because of its ability to rapidly colonize the roots of host plants and thus compete with soil borne pathogens for space and food. Present investigation was carried out to check deiversity of fluorescent Pseudomonas from rhizospheric and non-rhizospheric soil sample of Vadodara locality, Gujarat (India). In both rhizospheric and non-rhizospheric soils three species of fluorescent Pseudomonas were predominantly found viz., FP1 (yellow colony), FP2 (light yellow colony) and FP3 (green colony) and rhizospheric soil samples showed higher density of fluorescent bacteria (70.52-79.07) as compared to non rhizospheric soil samples (66.20-76.30). In present investigation total 10 isolates were obtained from rhizospheric and non-rhizospheric soil sample of Vadodara locality. Moreover the obtained isolates were further tested for their ability to control soil borne fungal pathogens viz., Fusarium oxysporum and F. udum causing wilt in crop plants. All the isolates were capable of restricting mycelial growth of soil borne wilt pathogen F. oxysporum and F. udum.