Vol. 1 (1) : January-February 2010 issue
Green Farming Vol. 1 (1) : 74-77 (January-February, 2010) (Renewed)
DNA polymorphism in sugarcane woolly aphid, Ceratovocuna lanigera Zehnter (Aphididae : Homoptera) through RAPD markers
P.V. MATTIa1*, C.P. MALLAPURa2, R.J. RABINDRAb3 and D.P. BIRADARa4
aDepartment of Agric. Entomology, University of Agricultural Sciences, Dharwad - 580 005 (Karnataka)
b Project Directorate of Biological Control (ICAR), Hebbal, Bangalore - 560 024 (Karnataka)
Designation : 1Research Scholar, 2Associate Professor (Ento.), 3Director, 4Editor
Subject : Entomology
Paper No. :
Total Pages : 4
Received :
Revised accepted :
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Citation :
P.V. MATTI, C.P. MALLAPUR, R.J. RABINDRA and D.P. BIRADAR. 2010. DNA polymorphism in sugarcane woolly aphid, Ceratovocuna lanigera Zehnter (Aphididae : Homoptera) through RAPD markers. Green Farming Vol. 1 (1) : 74-77 ; January-February, 2010 (Renewed)
ABSTRACT
RAPD technique helps to identify discreet genetic markers in Aphididae, a family in which biochemical and morphological genetic polymorphisms are rare. Investigations were carried out to study the genetic diversity through, molecular characterization of sugarcane woolly aphid (SWA) under laboratory conditions. RAPD revealed large amount of genetic variations among individuals. There exist variations in the DNA profiling among all samples. The dendrogram constructed using symmetric matrix of different samples resulted into two major clusters. The similarity matrix pertaining to different isolates of SWA revealed that the similarity coefficient ranged from 0.22-0.60. The highest genetic similarity index of 0.60 was seen between the isolates from Bangalore and Poona followed by 0.40 between the isolates from Assam, Sameerwadi (Bagalkot).
Key words :
Ceratovocuna lanigera, RAPD markers, Sugarcane, Wolly aphid.
