Field studies were conducted for two consecutive rabi seasons of 2011-12 and 2012-13 to study the effect of different bio-organics and fertilizer levels on nutrient uptake, yield and net returns of barley. Results revealed that among the different bio-organics, application of vermicompost @ 5 t/ha + Azotobacter + PSB resulted in significantly maximum increase in number of effective tillers, grains/spike, test weigh, total uptake of N, P and K over control. The increasing levels of fertilizers also significantly increased the yield attributes, total nutrient uptake and net returns in barley crop and significantly maximum improvement in yield attributes, nutrient uptake and net returns was noticed up to 75% RDF over control during both the years and in pooled. The combined application of vermicompost @ 5 t/ha + Azotobacter + PSB along with 75% RDF gave significantly higher grain yield of barley (4.79 t/ha) which was 66.1% higher over control. Whereas, significantly maximum net returns (` 49472/ha) were obtained from combined application of FYM @ 10 t/ha + Azotobacter + PSB with 75% RDF.