An experiment was conducted to find out the effect of defoliating chemicals (chemical defoliation) on growth and flower yield of Jasminum sambac .The main objective is to identify the effective chemical with maximum defoliation at low concentration without affecting plant growth and crop productivity, biologically safe, eco-friendly . The experiment consists of three local genotypes of Jasminum sambac (Nityamalli, Tuppamalli and Starmalli) as first factor, and three defoliant chemicals (viz., paraquat dichloride, sodium chloride and potassium nitrate) in total of 16 different treatment combinations along with control (water spray) taken as second factor replicated thrice in Factorial Randomized Block Design. The defoliant chemical paraquat dichloride @1000 and 2000 ppm has shown the maximum weight of defoliation (27.45g) minimum number of days for leaf emergence (10.23 days), minimum number of days for complete defoliation (1.23 days), high flower yield per plant (296.51 g) and highest aerial biomass of a plant (192.44 g). Among three genotypes Nityamalli responded well for paraquat dichloride with high cost benefit ratio of (1:1.56) compared to Starmalli and Tuppamalli. Thus it can be concluded that the tedious manual pruning operation can be replaced with the chemical defoliation with the use of defoliant chemicals such as paraquat dichloride which will be cost effective approach.