During kharif season of 2009-10 the effect of Minimum Tillage (MT), Conventional Tillage (PL), Subsoiling + Conventional Tillage(SS), Shallow Tillage by Tractor (STT) and Shallow Tillage by Bullock(STB) drawn on soil moisture has been studied in field experiment at the research farm of Agronomy, Dr. P.D.K.V. Akola. The five tillage treatments were evaluated for clay soil with split plot design under soybean (sole soybean) and cotton cropping system (cotton + blackgram, cotton+ pigeon pea). Treatment 'SS' (Subsoiling + Conventional Tillage) was found superior among all the tillage treatment in respect of moisture content values (24.11%) at depth of 0-15, 15-30 and 30-45 cm which was followed by treatment 'PL', 'STT', 'STB'. The minimum tillage (MT) treated plot conserved minimum moisture throughout investigation period. The moisture content was differed significantly in both cropping systems. However, cotton based was found to be non-significant. The significant interaction among the Tillage and Cropping system reflect that the level of soil manipulation and the canopy cover by crop are interrelated with each other. The investigations were concluded that very deep tillage like use of subsoiler to help in moisture conservation.