The distribution of mercury (Hg) among various fractions is desirable for better phytoextraction of Hg as water soluble and exchangeable fraction of Hg is highly available to the plants. Therefore, a screen house experiment (open air green house with natural environmental condition) was conducted with Brassica juncea grown on artificially spiked soil (40 µg Hg g-1) to study the effect of ethylene diamine tetraacetic acid (EDTA), farm yard manure (FYM), vermicompost (VC) and microbial inoculants (Azotobacter sp. and Pseudomonas sp.) on distribution of Hg in various fractions. A sequential extraction procedure (SEP) was used that fractionated Hg into water soluble (WS), exchangeable (Ex), organically bound (Org) and residual (Res) fractions. The fractionation of Hg revealed that application of chelating agent (EDTA) caused a significant increase in the water soluble and exchangeable fraction of Hg with a consequent decrease in organic fraction. Application of FYM and vermicompost significantly increased organically bound fraction of Hg but decreased other fractions. Application of bioinoculants was non-significant, moreover, they increased exchangeable and organically bound fraction of Hg either alone or in combination with FYM and VC. However, a great amount (80-85%) of the total Hg was present as residual Hg. So it can be concluded that even in the presence of chelating agents the solubility and mobility of Hg is low.