The present study was conducted to access the effect of different doses of gamma radiation on the storage performance of Kinnow fruits under ambient temperature conditions. Fruits were irradiated to gamma irradiation at different doses (75 Gy, 150 Gy, 225 Gy and 300 Gy). The control fruits were kept untreated and stored at room temperature for 0-21days. The observations on various physico-chemical quality attributes of fruits were recorded at different storage intervals. The results revealed that under ambient temperature storage, there was a significant increase in fruit physiological weight loss, TSS, reducing sugars and peel percentage and decay incidence with storage intervals. However, the other parameters like juice percentage, acidity and ascorbic acid contents were decreased. It is concluded that gamma irradiation dose @ 150 Gy was very effective in improving the overall quality and extending the shelf life of Kinnow fruits under ambient temperature stogie conditions.