Field experiment was conducted in red soils in order to study the Zn transformation in soil, yield and nutrient uptake using Arbuscular mycorrhizal (AM) fungi and Zinc solubilizing bacteria (ZSB) combination with graded levels of ZnSO4. Treatment consisted two factors viz., microbial inoculation (M1: control, M2: AM fungi, M3: ZSB and M4: M2+M3) and graded levels of ZnSO4 (S1 : 0, S2 : 12.5, S3 : 25, S4 : 37.5, S5 : 50 Kg ha-1 and S6 : 0.5% foliar spray @ 45 and 65 DAS) replicated three times in RBD. The results revealed that the microbial inoculation significantly increased the soil available Zn content at all stages of observation. Among the graded doses of Zn, the highest available Zn content was recorded with the treatment S5 followed by S4, S3 and S2 over the control. Increased availability of Zn in soil enhanced the maize grain and stover yield indicating response to added Zn under microbial inoculation. The Zn uptake by maize grain and stover also increased significantly for the treatment combinations.