The field experiment was undertaken to study the effect growing season on biochemical constituents and yield of orange fruited tomato cultivars under Rahuri conditions. The data revealed that, the growing season did not produce noticeable influence on the physical parameters of the fruit such as polar and radial diameter, pericarp thickness, no. of seeds per fruit and per cent juice content. Among biochemical traits, the similarity was also noticed in most of the parameters of orange and traditional red fruited cultivars except parameters like TSS, ascorbic acid and beta carotene content which was found to be more in orange fruited cultivars than the traditional red fruited one. However, the lycopene content was noticed more in red fruited cultivars than orange fruited ones. Among the seasons, not distinguished difference was noticed but rabi season had slightly more beta carotene (3.42 mg/100g) than the kharif season (3.20mg/100g). The significantly highest mean fruit yield was noticed in traditional red fruited cultivars (430.90 q/ha) than the orange fruited one (332.32 q/ha). The fruit yield was found to be more in rabi (452.21 q/ha) than kharif season (311.00 q/ha). It is concluded that the growing season did not produced significant influence on physic-chemical constituents. Among biochemical traits,