The present paper reports the 59 years, long-term effect of organic manure, fertilizer and lime on available plant nutrient in soil, changes observed in available phosphorus (P) content of soil at critical growth stages of wheat, uptake of P in wheat crop and crop yields under maize-wheat cropping sequence in an acid soil. The pH of an acid soil increased by 0.8 units with lime and fertilizer use. FYM, manure treated plots had a favourable influence on both soil pH and organic carbon content. The highest available P was recorded in continuous addition of NPK (595 kg ha-1) which was significantly at par with NP (581 kg ha-1) and was significantly superior over others. Marginal changes observed in available P during critical growth stages of wheat in all treatments. However available P maintained its higher available P status throughout the wheat crop growth period. The maximum water soluble phosphorus (16.59 mg kg-1) was recorded in FYM+ NPK treated plot which was significantly at par with FYM (14.25 mg kg-1 P) over all the other treatments. Continuous application of NP (imbalance major nutrients) resulted in the higher total content of P which was statistically superior to all the treatments The highest P uptake was noted in NPK + lime treated plots (22.2 kg ha-1) followed by FYM+ NPK (20.2 kg ha-1). Results revealed that highest grain yield of maize (35.3 q ha-1) and wheat (35.7 q ha-1) were obtained due to NPK+ Lime which was on par with the yield obtained with INM treatment, NPK in combination with FYM maize (32.3 q ha-1) and wheat (29.8 q ha-1). Continuous use of N alone had a depression effect on soil pH (3.7) and led to lowest grain yield in both the crops.