ABSTRACT . Cultivation practices such as tillage, imbalanced fertilizer application coupled with repeated mulberry biomass harvests exposes the soil to the vagaries of weather and affecting soil quality. A study was under taken to evaluate organic carbon, microbial biomass carbon (Cmic) and nitrogen (Nmic), dehydrogenase enzyme activity (DHA) soil porosity, water holding capacity (%) and soil bulk density as soil quality indicator for practices like combined application of organic and inorganic fertilizer with tillage (T0), crop residue management and organic in put application with no –tillage (T1) and chemical fertilizer with tillage (T2) respectively at Central Sericulture Research and Training Institute, Mysore (2008-2010). Results indicated that total soil organic carbon was significantly increased in T1 (0.85%) > T0 & T2 in which it was 0.69%. Similarly, microbial biomass carbon (Cmic) was 2.09 X 10-2 (gm-1 soil) in T1 > T0 (1.54 X 10-2 (gm-1 soil) > T2 (0.98 X 10-2 (gm-1 soil) and microbial biomass nitrogen (N-mic ) was also shown the same trend as in Cmic. It was high in T1 (2.44 X 10-3 (gm-1 soil) > T0 (1. 79 X 10-3 (gm-1 soil) > T2 (1.14 X 10-3 (gm-1 soil). With increasing Corg /SOC, Cmic, and Nmic, dehydrogenase enzyme activity was also increased in T1 (2.78mg TPR-1hr-1 ) followed by T0 and T2. DHA often using as a measure of any change in the soil management and important as it gives indications for soil potentiality to support for the biochemical process, which are essential for maintaining soil fertility. Soil bulk density was significantly reduced in T1 (1.20gm/cc) < T0 (1.25gm/cc) < T2 (1.27gm/cc). Soil porosity and water holding capacity of the soil was high in T1 when compared T0 and T2. Regression analysis revealed that SOC was significantly and positively associated in increasing soil porosity and water holding capacity by showing high regression co-efficient values (r2 > 85%). Soil bulk density was negatively correlated in its reduction with increasing SOC in T1. Further regression analysis of pooled data have shown that Corg was significantly and positively associated in Cmic and Nmic by showing high regression coefficient. DHA showing an increasing trend with increasing Corg and Nmic, The study suggests crop residue management; no-till and organic input application practices that sequester carbon efficiently to maintain the soil fertility can be advocated as an important agronomical strategy for the sustainable crop and environmental development.