An experiment was conducted at College Agronomy Farm, B.A. College of Agriculture, Anand Agricultural University, Anand rabi seasons to study the effect of organic and inorganic fertilizers in comparison with humic acid on quality of wheat (Triticum aestivum L.) on nutrient content and nutrient status of sandy loam soil. There were total eighteen treatment combinations with four replication tested under Randomized block design (Factorial). Results revealed that application of Recommended dose of fertilizer (120+60+0 NPK, kg/ha) treatment was recorded significantly the highest protein content (11.35%), nitrogen content in grain (1.815 %), stover (0.365 %), phosphorus content in grain (0.372 %) and stover content (0.103 %) as well as micronutrient content, Fe (282.30 mg/ha) and Zn content (16.312 mg/ha), after harvest soil nutrients content viz. available nitrogen (346.70 kg/ha), available phosphorus (55.33 kg/ha) and micronutrient status Fe (6.71 mg/ha), Cu (1.214 mg/ha), while Zn (0.796 mg/ha) was found the highest in F3 (50 % recommended dose of fertilizer (60+30+0 NPK, kg/ha) treatment. Organic manure treatment M4 (Farm yard manure @5 t/ha + Humic acid (Soil application @10 kg/ha + Foliar application @ 0.1%) recorded significantly higher protein content (10.99 %), nitrogen content in grain (1.759 %), phosphorus content in grain (0.349 %), straw (0.083 %) and available nitrogen content in soil (343.90 kg/ha). Application of micro nutrients (Soil application of Government notified grade-V @20 kg/ha) (M6) was recorded significantly higher Fe (279.70 mg/kg), Mn (23.949 mg/kg), Zn (16.214 mg/kg) and significantly the highest cu (4.384 mg/kg) content in grain. Significantly higher nitrogen content in stover (0.336 %) was noted in M3 (FYM @10 t/ha) treatment. Treatment M4 recorded significantly higher available nitrogen content (343.90 kg/ha) in soil after harvest of crop. Significantly higher Fe (6.99 mg/kg) and the highest Zn (0.813 mg/kg) and Cu (1.208 mg/kg) was noted in treatment M6 Micro nutrients. Interaction effects of inorganic fertilizers and manuers/fertilizer found significant on protein content, nitrogen content in grain and stover, phosphorus content in stover, Zn content in grain, available nitrogen content and Zn content in soil after harvest of crop.