A trial was conducted at Department of Horticulture, Junagadh Agricultural University, Junagadh to investigate the effect of organic, inorganic and biofertilizer on growth, yield and quality of ratoon tuberose cv. Double. Sixteen treatment combinations of different nutritional sources were comprised with three replications. Results due to various treatments found significant. Application of FYM @ 30 t/ha + PSB @ 2 g/m2 + Azoto. @ 2 g/m2 (T13) was recorded for minimum days to sprouting (18.47 days), maximum plant height (61.67 cm) and plant spread at E-W and N-S (37.93 cm and 37.07 cm, respectively). In case of flowering, significantly maximum length of spike (78.00 cm), number of florets per spike (44.07), number of spikes per plant (4.26), number of spikes per net plot (127.67), number of spikes per hectare (4.73 lacks), longest vase life (12.33 days) and in situ longevity of spike (20.80 days) were recorded in treatment ½ RDF + NC @ 1 t/ha + PSB @ 1 g/m² + Azoto.@ 1 g/m²(T4). Similarly, the application of ¼ RDF + PSB @ 1.5 g/m2+ @ Azoto.1.5 g/m2 (T6) gave poor performance.