Vol. 7 (1) : January-February 2016 issue
Green Farming Vol. 7 (1) : 161-164 ; January-February, 2016
Effect of phosphate concentration on the production of antho- cyanins in the cell suspension cultures of grape (Vitis vinifera L.)
R.A. BHIRANGIa1*, J.M. KHILARIa2 and U.J. MEHTAb3
aMaharashtra State Grape Growers Association, Manjri Farm PO, Pune – 412 307 (Maharashtra), bPlant Tissue Culture Division, CSIR-National Chemical Laboratory, Pune - 411 008 (Maharashtra)
Designation : 1Sr. Chemist *(ritu.bhirangi81@gmail.com), 2Ex-President, 3Scientist-F
Subject : Fruit Science and Pomology
Paper No. : P-3361
Total Pages : 4
Received : 20 February 2015
Revised accepted : 30 December 2015
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Citation :
R.A. BHIRANGI, J.M. KHILARI and U.J. MEHTA. 2016. Effect of phosphate concentration on the production of antho-cyanins in the cell suspension cultures of grape (Vitis vinifera L.). Green Farming Vol. 7 (1) : 161-164 ; January-February, 2016
ABSTRACT
The influence of phosphate on the accumulation of anthocyanins and the production of the cell biomass was investigated in the cell cultures of Vitis vinifera cv Thompson Seedless during the year 2011-12. MS basal media supplemented with BAP and NAA yielded maximum callus induction and proliferation. It was indicated that the anthocyanin content increased with the decrease in phosphate concentration. The highest anthocyanin content (5.487mg/l) was recorded at 0.321mM phosphate concentration. However, a further decrease in phosphate concentration resulted in still lower anthocyanins, the reason of which needs to be investigated separately. At the highest phosphate concentration (2.5mM) the anthocyanin content was the least. The cell biomass was also observed to be influenced by the phosphate concentration. It increased with the decrease in phosphate concentration from 2.5mM to 0.321mM but decreased at 0.165 mM phosphate concentration, at which it was found to be the limiter for biomass production and hence the anthocyanin content.
Key words :
Anthocyanins, Cell biomass, Grape, Phosphate concentration, Thompson Seedless, Vitis vinifera.
