An experiment was carried out at the Medicinal and Aromatic Plants Research Farm, Anand Agricultural University, Anand (Gujarat) to study the effect of planting period and spacing on growth, biomass, yield and quality of jamarosa (Cymbopogon jwarancusa) under middle Gujarat condition during the kharif-rabi seasons of the year 2010-11. The experiment consisted of three treatments of dates of planting (D1: 1st June, D2:1st July and D3: 1st August) relegated to main plots as main plot treatment and three spacings (S1: 45x30 cm, S2: 60x30 cm and S3: 75x30 cm) as sub-plot treatments in sub plots. Thus, there were nine treatment combinations tested in the split plot design with three replications. The results recorded that the growth attributes have significantly influenced due to dates of planting and recorded the maximum with the planting under 1st June over other dates of planting. The herbage and oil yield recorded significantly higher under planting 1st June over 1st August, However, it remained at par with 1st July. Spacings had significant effect on majority of the growth and yield attributes. Among three spacings, 45 x30 cm gave good response. 45x30cm recorded significantly higher plant height, length of leaves, fresh and dry herbage yield over the treatment of 60x30 cm and 75x30 cm at all the cuttings. Whereas, the planting at 75x30 cm spacing produced significantly higher average number of tillers plant-1 and oil content (%) as compared to treatments 45x30 cm and 60x30 cm spacings under three cuttings, respectively. It is concluded that jamarosa can be advised to plant either on 1st June or 1st July with followed either spacing of 45x30 cm or 60x30 cm or 75x30 cm for securing higher oil yield and economic return.