Vol. 5 (5) : September-October 2014 issue
Green Farming Vol. 5 (5) : 768-771 ; September-October, 2014
Effect of pollen viability and method of storage on seed set, seed yield and seed quality under in-vivo and in-vitro in sunflower variety RHA-857
KANTHARAJU1, PRIYA KIVADASANNAVAR2* and V.K. DESHPANDE3
Department of Seed Science and Technology, University of Agricultural Sciences, Dharwad - 580 005 (Karnataka)
Designation : 1Agric. Officer, 2Res. Assoc. *(priya.bk@rediffmail.com), 3Associate Professor
Subject : Seed Science Technology
Paper No. : P-1283
Total Pages : 4
Received : 10 February 2014
Revised accepted : 12 September 2014
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Citation :
KANTHARAJU, PRIYA KIVADASANNAVAR and V.K. DESHPANDE. 2014. Effect of pollen viability and method of storage on seed set, seed yield and seed quality under in-vivo and in-vitro in sunflower variety RHA-857. Green Farming Vol. 5 (5) : 768-771 ; September-October, 201
ABSTRACT
In vivo pollen viability studies revealed that there was no significant difference with 100 percent fresh pollen (61.98) and combination of 75 percent pollen + 25 percent mixture and same either as fresh or stored in refrigerator and earthen pot for one day. Application of pollens stored under ambient condition for one day it was resulted in poor seed set i.e. 41.07 to 48.16 percent. Seed yield as measured setting percentage and 100 seed weight were maximum with cent percent pollen either as fresh or refrigerator and earthen pot storage. Lower values recorded in pollen stored at ambient condition for one day. In-vitro studies on pollen viability indicated that pollens were very susceptible to storage under ambient conditions when compared to pollen stored in refrigerator or moist earthen pot. Pollens stored in refrigerator even up to two days were equally germinable with better pollen tube length compared to that of pollen stored for one day in earthen pot. The addition of filler material and boron (99 g of rice flour and 1g of boron) at different proportions with the fresh and stored pollens in different storage conditions revealed that the 75 percent pollen and 25 percent filler mixture combination recorded equal performance as that of 100 percent pollen exhibiting better pollen germination and better pollen tube growth compared to 50 percent pollen and 50 percent mixture.
Key words :
Filler mixture, In vitro, Pollen viability, Pollen storage, Sunflower.