Present research experiment was designed to evaluate the impact of post-harvest treatments of CaCl2, CaNO3 (at 1% and 2%, each), SA and NAA (100 ppm and 200 ppm, each) on storage behaviour and quality of cv. Allahabad Safeda guava fruits during ambient and cold storage. These chemicals were applied to uniform coloured and same size fruits of Allahabad Safeda guava fruits by dipping method for 3 minutes. After wiping they were packed in CFB boxes (4 kg capacity and sized, 32 x 16 x 16 cm) and kept under ambient as well as cold storage conditions (8-10oC, 90-95 % RH) and analyzed at interval of 0, 3, 6 and 9 days ambient stored whereas for cold storage conditions, interval was 0, 7, 14 and 21 days, for physio-chemical characters. It can be concluded from current research experiment that cold conditions are much better as compare to ambient storage and guava can be stored for 6 days (under ambient) and 14 days (under cold conditions), respectively with minimum loss in quality.