A field experiment was conducted in 2013-14 to investigate effect of different levels of potassium and sulphur on content and uptake of nutrients (N, P, K and S) in different plant parts, at different growth stages of popcorn (Zea mays L. Everta Sturt) at the Instructional Farm, College of Agriculture, Junagadh Agricultural University, Junagadh during rabi season. Factorial Randomized Block Design replicated three times was tested in the experiment. Four levels each of potassium viz., 0, 30, 60 and 90 kg K2O ha-1 and sulphur viz., 0, 20, 40, 60 kg S ha-1 were used. Among these levels potassium @ 60 kg K2O ha-1 (K3) produced significant effect on concentration of potassium in leaves, shoot and root at 30, 60 DAS as well as in leaves, shoot, root and seeds of popcorn at harvest. And also application of sulphur @ 40 kg S ha-1 (S3) produced significant effect on concentration of sulphur in leaves, shoot and root at 30, 60 DAS and significant effect on N concentration in leaves at 60 DAS as well as significant effect on leaves, shoot, root and seeds of popcorn at harvest. Application of potassium @ 60 kg K2O ha-1 and sulphur @ 40 kg S ha-1 gave significantly higher uptake of (N, P, K and S) by leaves, shoot and root at 30, 60DAS and at harvest over that of lower potassium and sulphur levels. Similarly, the highest uptake of (N, P, K and S) by seed recorded significantly at potassium applied @ 60 kg K2O ha-1 (K3) and sulphur applied @ 40 kg S ha-1.