A research trial was conducted to study the effect of pre-harvest application of plant growth regulators and different packaging materials on shelf-life of custard apple (Annona squamosa L.). The three plant growth regulators i.e. GA3 (40 and 50 ppm), NAA (20 and 30 ppm) and 2, 4-D (10 and 20 ppm) were applied as pre-harvest and fruits were packed in three different packaging materials (bamboo basket, corrugated boxes and plastic crates).The various physico–chemical observations were recorded on 0th, 2nd, 4th, 6th and 8th day during storage. The results revealed that pre-harvest plant growth regulators and packaging were found to be significant on maintaining the shelf-life of custard apple fruits during storage. Among the treatments, the pre-harvest application of 2,4-D 20 ppm and fruits packed in corrugated boxes (treatment T17) recorded higher shelf-life and retained better quality of fruit with respect to maximum firmness, TSS, ascorbic acid, pH and total sugar, reducing sugar and non-reducing sugar contents with low acidity up to the 8th day of storage. The minimum PLW of fruits was also recorded in treatment T17.