The field experiment was carried out using banana as a test crop during 2012-2013 (plant) and 2013-2014 (ratoon) at Soil and Water Management Research Unit, NAU, Navsari. Nutrients viz., Fe, Mn, Zn and Cu content and uptake at harvest by leaves, pseudostem and fruits were found to increases in treatment T7 (T1+M3S2) in both plant and ratoon banana compared to control. The uptake pattern of Fe, Mn, Zn and Cu was similar to that observed with dry matter yield and fruit yield of banana crop of both the years (plant and ratoon). From the distribution point of view, most of the nutrients were accumulated more in pseudostem than fruit and leaves and it was pronounced in the case of Fe uptake. In plant banana (2013-14) the Mn content in fruit, leaf and pseudostem is 72.17, 440.36 and 196.41 mg kg-1 respectively, in plants treated with T7 treatment. Mn uptake was 53%, 44% and 45% more in T7 treatment as compared to control in 2012-13(plant banana) in fruit, leaf and pseudostem respectively. The treatment T6 was found at par with treatment T7 in content and uptake of micronutrient viz., Fe, Mn, Zn and Cu in banana fruit, leaf and pseudostem. The iron content was more in the pseudostem followed by leaves and fruits. On contrary, the zinc and manganese contents were higher in the pseudostem followed by and fruits and leaves. The micronutrient contents were in the order of Mn>Fe>Zn in the leaves, Fe>Mn>Zn in the pseudostem and Fe>Mn>Zn in the fruits. Therefore, it was concluded that micronutrient application in soil through different rate and frequency brought improvement nutrient content and uptake in different parts of plant.