Mulberry (Morus alba L.) is vegetatively propagated through stem cuttings and these cuttings are directly planted in main field or saplings are raised in nurseries. The cuttings are exposed to various soil-borne pathogens of which stem canker caused by Lasiodiplodia theobromae is more serious in recent times causing 30-70% mortality of cuttings. The effect of fungicides was tested against the pathogen through spore germination and poison food technique at 0.1, 0.2 and 0.3 % concentrations to identify the effective fungicide under in vitro conditions. A total of five fungicides (systemic and contact) viz., Bavistin (carbendazim 50% WP), Ridomil MZ Gold (metalaxyl), Kocide (copper hydroxide 77% WP), Indofil M-45 (mancozeb 75%WP), and Blitox (copper oxychloride 50% WP) were screened. Among the fungicides tested carbendazim, metalaxyl and copper hydroxide recorded cent percent inhibition of spore germination at all the concentrations tested. Whereas mancozeb and copper oxychloride were less effective. Further, the mycelial growth of the test pathogen was completely inhibited by carbendazim, copper hydroxide and metalaxyl at all the concentrations tested and found to be highly effective and promising. Whereas mancozeb was found moderately effective at 0.2 % concentration onwards. The effective fungicides can be used for the management of stem canker disease in mulberry nurseries.