Zinc fertilization in bread wheat cultivated in vertisols during rabi 2012 enhanced the leaf zinc content at pre-anthesis and post-anthesis stage and the grain zinc content at harvest and also the grain yield. Among the different treatments, foliar application of zinc sulphate (0.5%) at 60 DAS contributed more in increasing leaf zinc content compared to foliar application at 30 DAS and soil application of zinc sulphate (10kg ha-1). The activities of oxidative enzymes such as Peroxidase (POX) and Superoxide Dismutase (SOD) in flag leaf at 5 days after anthesis differed significantly among zinc treatments (soil and foliar application). The POX and SOD activity also varied significantly among germplasm lines. The grain yield was higher in foliar application (2685 kg ha-1) followed by soil application (2568 kg ha-1) and significantly lower in control (2383 kg ha-1). The germplasm lines viz., 554, 606 and 636 have higher grain zinc content, whereas higher yield was recorded in local check Lok-62, but has low zinc compared to other lines. Germplasm lines viz., 127, 606 and 636 maintained fairly good amount grain zinc content without compromising the yield. It is concluded that the zinc fertilization has a vital role in both crop growth and enhancing the grain zinc content which ultimately help crops to mitigate zinc deficiency and also to alleviate the micronutrient malnutrition in human beings caused by zinc insufficiency.