Field experiment was conducted during kharif season of 2013-14 at Research farm, Department of Soil Science and Agricultural Chemistry, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani on Vertisol to evaluate the effect of these selected microbial strains on yield, nutrient uptake and quality parameters of Bt cotton. Total eight treatments of microbial cultures were replicated three times in Randomized Block Design. The results emerged out indicated that Trichoderma viride and Pseudomonas striata along with RDF were found better strains in increasing seed cotton yield and dry matter yield. Significantly highest uptake of major nutrients were recorded in the treatment receiving Trichoderma viride, Pseudomonas striata and Burkholderia cepacia. Among the microbial strains, fungal strain Trichoderma viride was found significantly superior in increasing total zinc uptake by cotton stalk followed by Pseudomonas striata and Burkholderia cepacia. Quality parameters of cotton fiber like ginning percentage, lint index and oil + protein content of cotton seed were also improved over only inorganic fertilizer applied plots and numerically over other microbial strains.